Website Creighton University

We are looking for a detail-oriented and dedicated Lab Technician with expertise in genotyping, mouse handling, colony management, and histology to support our research efforts. The successful candidate will play a crucial role in ensuring the smooth operation of our laboratory and the success of our research projects.

The lab is equipped for modern molecular biology experiments, advanced mRNA and protein spatial distribution analysis using RNA scope and PLA assays, in vitro tests with organ cultures, and in vivo investigations with live animals. Utilizing the state-of-the-art electrophysiologic and genetic techniques combined with microscopy, the Renauld lab is focusing on the development and regulation of the stria vascularis to investigate dysfunction in inner ear homeostasis. Our current projects focus on the regulation of the endolymph and identify how stress hormones influence endolymph regulation as well as the mechanism leading to neuronal loss in Meniere’s disease. Results of this proposed work will advance our understanding of Meniere’s disease and direct us toward the development of treatments to alleviate symptoms or prevent neuronal death.

 

Key Responsibilities:

Genotyping PCR:
Perform genotyping PCR assays to analyze DNA samples.
Maintain accurate records of genotyping results.
Troubleshoot and optimize PCR protocols as needed.
 

Mouse Handling and Colony Management:

Monitor and maintain mouse colonies, including breeding and record-keeping.
Assist in experimental procedures involving mice, such as tagging or tissue collection.
 

Histology:

Prepare tissue samples for histological analysis (tissue cryosection, immunostaining).
 

Electrophysiology:

Auditory brainstem response (ABR) and Vestibular sensory-evoked potential (VsEP) recording.
Possibility to learn endocochlear potential recording.
 

Lab Maintenance:

Oversee inventory of supplies necessary for daily laboratory work.
Prepare solutions, set up reactions. Maintain laboratory equipment.
 

Data Management:

Maintain detailed and organized records of experimental data.
Assist in data analysis and contribute to research reports.
 

Collaboration:

Work closely with researchers and other team members to achieve project objectives.
 

Qualifications:

 

Bachelor’s degree in a relevant scientific field (e.g., biology, genetics, molecular biology) or equivalent.
Expertise in genotyping PCR techniques.
Proficiency in mouse handling and colony management.
Experience in histological sample preparation and analysis.
Excellent attention to detail and record-keeping skills.
Ability to work independently and as part of a team.
Problem-solving and troubleshooting abilities.
Effective communication skills.

To apply for this job please visit creighton.referrals.selectminds.com.